Analyzing yeast colonies by PCR.
نویسندگان
چکیده
MATERIALS Taq DNA polymerase Marker DNA As a reference, use single-stranded DNA from the original bacteriophage M13 recombinant. MgCl2 (25 mM) Oligonucleotide primers The oligonucleotides should be 20-24 nucleotides in length, specific for the target DNA sequences, free of potential secondary structures, and contain no less than 10 and no more than 15 G and C residues. Yeast colony PCR buffer, 10x Yeast strain carrying recombinant YAC of interest dNTP solution (10 mM) containing all four dNTPs (pH 8.0; PCR grade)
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ورودعنوان ژورنال:
- CSH protocols
دوره 2006 1 شماره
صفحات -
تاریخ انتشار 2006